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The Journal of Adhesive Dentistry



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J Adhes Dent 17 (2015), No. 4     10. Sep. 2015
J Adhes Dent 17 (2015), No. 4  (10.09.2015)

Page 347-352, doi:10.3290/j.jad.a34594, PubMed:26295068

Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength
Perote, Letícia C. C. Costa / Kamozaki, Maria Beatriz Beber / Gutierrez, Natália C. / Tay, Franklin R. / Pucci, Cesar R.
Purpose: This study examined the effects of matrix metalloproteinase-inhibiting solutions and aging methods on the bond strength between resin composite and human dentin.
Materials and Methods: Crown segments of 105 human non-carious molars were bonded using simulated pulpal pressure at 20 cm water pressure. The teeth were randomly split into 5 groups according to the solution applied: CG (control, no solution), CHX (0.2% chlorhexidine), EPE (10% ethanolic propolis extract), APE (aqueous propolis extract), and E (70% ethanol). Each solution was left on the acid-etched dentin for 1 min. Adper Single Bond 2 and resin composite (Filtek Z350 XT) were applied to all specimens. The 5 groups were subdivided according to the aging method: SI (sectioned immediately); S (storage in artificial saliva for 6 months); and T (thermomechanical aging with 240,000 mechanical cycles and 1000 thermal cycles). Specimens were sectioned into sticks and subjected to microtensile testing. Bond strength data were analyzed by two-factor ANOVA followed by a post-hoc Tukey's test (α = 0.05).
Results: For the factor "solution", there was no significant difference among the groups (p = 0.32). For the factor "aging method", significant differences were found (p < 0.001), with the following mean bond strengths (MPa): SI: 31.1; S: 24.4; T: 26.8.
Conclusions: The use of matrix metalloproteinase-inhibiting solutions on dentin as an adjunct to the application of an etch-and-rinse adhesive does not prevent the loss of bond strength after aging. Nevertheless, these solutions have no adverse effect on adhesion to tooth structure.

Keywords: aging, matrix metalloproteinase, resin composite, tensile strength