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The Journal of Adhesive Dentistry
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J Adhes Dent 17 (2015), No. 2     12. May 2015
J Adhes Dent 17 (2015), No. 2  (12.05.2015)

Page 155-161, doi:10.3290/j.jad.a33892, PubMed:25763410


Responses of Dental Pulp Cells to a Less Invasive Bleaching Technique Applied to Adhesive-Restored Teeth
Soares, Diana Gabriela / Sacono, Nancy Tomoko / Ribeiro, Ana Paula Dias / Basso, Fernanda Gonçalves / Scheffel, Débora Sales / Hebling, Josimeri / Costa, Carlos Alberto de Souza
Purpose: To assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin.
Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%).
Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time.
Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.

Keywords: laboratory research, toxicity, odontoblasts, tooth bleaching, adhesives