We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
The Journal of Adhesive Dentistry



Forgotten password?


J Adhes Dent 15 (2013), No. 6     15. Dec. 2013
J Adhes Dent 15 (2013), No. 6  (15.12.2013)

Page 541-546, doi:10.3290/j.jad.a29588, PubMed:23593641

An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
Rodriguez, Ismael A. / Ferrara, Carlos A. Rozas / Campos-Sanchez, Fernando / Alaminos, Miguel / Echevarría, Jorge Uribe / Campos, Antonio
Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA).
Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used.
Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures.
Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts.

Keywords: glass-ionomer cements, biocompatibility, intracellular, electron probe microanalysis, human gingival fibroblast, LDH
fulltext (no access granted) order article as PDF-file (20.00 €)